FIGURE 2.7. Half-images originating from the same specimen.


Half-images originating from the same specimen. The left side represents the CPF channel and the right side does the YFP channel from the image splitter. The image shown is one of a whole image series taken during the experiment introduced in this chapter. A gray scale table was used, ranging from 767 to 12,650 arbitrary units (a.u). The cell in the YFP channel shows a higher intensity than in the CFP channel. This is caused by the cross talk between CFP intensity in the YFP channel.

From: Chapter 2, Monitoring Receptor-Mediated Changes of Intracellular cAMP Level by Using Ion Channels and Fluorescent Proteins as Biosensors

Cover of Serotonin Receptors in Neurobiology
Serotonin Receptors in Neurobiology.
Chattopadhyay A, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

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