FIGURE 2.6. FRET analysis results from the experiments introduced in this chapter.

FIGURE 2.6

FRET analysis results from the experiments introduced in this chapter. CFP and YFP intensities were measured from a neuroblastoma cell N1E-115 transfected with Epac and 5-HT7 receptor. (A) Region of interest (blue) drawn around the cell as well as the background region (red) used for background correction. (B) Normalized intensity traces of CFP (black) and YFP (red) resulting from Equation 2.12. The respective CFP to YFP ratio calculated with Equation 2.13 is shown as a green trace. A dramatic effect due to intracellular cAMP increase is visible after application of the 5-HT7 receptor agonist 5-CT. An air bubble arriving from the solution application system and resting underneath the objective reduces the intensity of the CFP and YFP traces to the same fraction. Therefore, it has no influence in the trace showing CFP to YFP ratio.

From: Chapter 2, Monitoring Receptor-Mediated Changes of Intracellular cAMP Level by Using Ion Channels and Fluorescent Proteins as Biosensors

Cover of Serotonin Receptors in Neurobiology
Serotonin Receptors in Neurobiology.
Chattopadhyay A, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

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