From: Applications of Clinical Microbial Next-Generation Sequencing
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Applications of Clinical Microbial Next-Generation Sequencing: Report on an American Academy of Microbiology Colloquium held in Washington, DC, in April 2015. Washington (DC): American Society for Microbiology; 2016. doi: 10.1128/AAMCol.Apr.2015
Applications of Clinical Microbial Next-Generation Sequencing: Report on an American Academy of Microbiology Colloquium held in Washington, DC, in April 2015.
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Figure 4.Illumina Genome Analyzer sequencing, the first of the Illumina platforms to be commercially launched.
Adapter-modified, single-stranded DNA is added to the flow cell and immobilized by hybridization. Bridge amplification generates clonally amplified clusters. Clusters are denatured and cleaved; sequencing is initiated with addition of primer, polymerase (POL) and 4 reversible dye terminators. Postincorporation fluorescence is recorded. The fluor and block are removed before the next synthesis cycle.
Republished from reference 5 with permission from the publisher.
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