Table 2PubChem BioAssays

Assay Brief NameAssay NameAssay TypeTargetPowder SampleCompound Concentration
Maybridge 16K library: primary screen AID-1792Primary cellular high-throughput screening assay to measure NOX1 inhibitionPrimary Assay (1X% INH)NOX1No7 μM
Maybridge 16K library: counterscreen screen Summary AID-1796Primary biochemical high-throughput screening assay to measure H202- luminol inhibitionPrimary Assay (1X% INH)H2O2No7 μM
Maybridge 16K Confirmation screen Summary AID-1796Confirmation cellular high- throughput screening assay to measure NOX1 inhibitionConfirmation Assay (triplicate) CounterscreenNOX1No7 and 3.3 μM
Cytoxicity Summary AID-1796Cellular CytotoxicityAssay (triplicate) ConfirmationCellular ATPNo7 μM
NOX1 293 confirmation Summary AID-1796Confirmation screening cellular assay to measure NOX 1 inhibitionAssay (3X% INH)NOX1Yes100 μM
NOX1 293 dose response Summary AID-1796Dose response cellular assay to measure NOX 1 inhibitionDose Response AssayNOX1Yes5-point, 1:4 dilution starting at 33 μM
NOX2 293 counterscreen Summary AID-1796Counterscreening cellular assay to measure NOX 2 inhibitionCell-based Low Throughput AssayNOX2Yes10 μM
NOX 3 293 counterscreen Summary AID-1796Counterscreening cellular assay to measure NOX 3 inhibitionCell-based Low Throughput AssayNOX3Yes10 μM
NOX 4 293 Summary AID-1796Counterscreening cellular assay to measure NOX 4 inhibitionCell-based Low Throughput AssayNOX4Yes10 μM
Xanthine Oxidase Summary AID-1796Counterscreen for inhibitors of Xanthine OxidaseBiochemical AssayXanthine OxidaseYesDose response

From: Probe Report for NOX1 Inhibitors

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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