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Alberts B, Bray D, Lewis J, et al. Molecular Biology of the Cell. 3rd edition. New York: Garland Science; 1994.

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Cover of Molecular Biology of the Cell

Molecular Biology of the Cell. 3rd edition.

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Figure 4-45. Two-dimensional polyacrylamide-gel electrophoresis.

Figure 4-45Two-dimensional polyacrylamide-gel electrophoresis

All the proteins in an E. coli bacterial cell are separated in this gel, in which each spot corresponds to a different polypeptide chain. The proteins were first separated according to their isoelectric points by isoelectric focusing from left to right. They were then further fractionated according to their molecular weights by electrophoresis from top to bottom in the presence of SDS. Note that different proteins are present in very different amounts. The bacteria were fed with a mixture of radioisotope-labeled amino acids so that all of their proteins were radioactive and could be readily detected by auto-radiography (see p. 180). (Courtesy of Patrick O'Farrell.)

From: Fractionation of Cells and Analysis of Their Molecules

Copyright © 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D Watson.

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