Figure 167.6. Double immunodiffusion by the Ouchterlony method.

Figure 167.6

Double immunodiffusion by the Ouchterlony method. The center well containing calf thymus nuclear extract (CTE) is surrounded by wells into which prototype serum with specificity for RNP (P) or patients" serum (1–4) has been placed. The precipitin lines represent antigen–antibody complexes. The line formed by serum 3 is identical with that of the prototype line (anti-RNP), but those formed by serum from patients 1 and 4 cross the adjacent lines and are nonidentical, indicating other antigen–antibody reactions.

From: Chapter 167, Immunologic Tests

Cover of Clinical Methods
Clinical Methods: The History, Physical, and Laboratory Examinations. 3rd edition.
Walker HK, Hall WD, Hurst JW, editors.
Boston: Butterworths; 1990.
Copyright © 1990, Butterworth Publishers, a division of Reed Publishing.

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