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Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002.

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Molecular Biology of the Cell. 4th edition.

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Table 8-1Composition of a Typical Medium Suitable for the Cultivation of Mammalian Cells

AMINO ACIDSVITAMINSSALTSMISCELLANEOUSPROTEINS (REQUIRED IN SERUM-FREE, CHEMICALLY DEFINED MEDIA)
ArgininebiotinNaClglucoseinsulin
CystinecholineKClpenicillintransferrin
GlutaminefolateNaH2PO4 streptomycinspecific growth factors
HistidinenicotinamideNaHCO3 phenol red
IsoleucinepantothenateCaCl2 whole serum
LeucinepyridoxalMgCl2
Lysinethiamine
Methionineriboflavin
Phenylalanine
Threonine
Trytophan
Tyrosine
Valine

Glucose is used at a concentration of 5–10 mM. The amino acids are all in the L form and, with one or two exceptions, are used at concentrations of 0.1 or 0.2 mM; vitamins are used at a 100-fold lower concentration, that is, about 1 μM. Serum, which is usually from horse or calf, is added to make up 10% of the total volume. Penicillin and streptomycin are antibiotics added to suppress the growth of bacteria. Phenol red is a pH indicator dye whose color is monitored to ensure a pH of about 7.4. Cultures are usually grown in a plastic or glass container with a suitably prepared surface that allows the attachment of cells. The containers are kept in an incubator at 37°C in an atmosphere of 5% CO2, 95% air.

From: Isolating Cells and Growing Them in Culture

Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .

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