FIGURE 18.4. A real microelectrode near a real cell.


A real microelectrode near a real cell. This figure illustrates a Ca2+ ISE located at the near pole position for measurement from a cultured INS-1 cell. As the electrode is silanized, and the cocktail lipophilic, stability of electrode placement and movement is critical. The electrode must not touch the cell. Control of motion is discussed in “Equipment and Software”.

From: Chapter 18, Principles, Development and Applications of Self-Referencing Electrochemical Microelectrodes to the Determination of Fluxes at Cell Membranes

Cover of Electrochemical Methods for Neuroscience
Electrochemical Methods for Neuroscience.
Michael AC, Borland LM, editors.
Boca Raton (FL): CRC Press/Taylor & Francis; 2007.
Copyright © 2007, Taylor & Francis Group, LLC.

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