Table 1Suggested modifications of standard methods for susceptibility testing of H. pylori

ParameterUSA-NCCLSEuropean H. pylori Study Group
MethodAgar dilutionAgar dilution
MediumMueller-Hinton agar plus aged (≥2 weeks old) sheep blood (5% vol/vol)Mueller-Hinton agar plus horse blood (10% vol/vol)
Inoculum1 × 107–1 × 108 CFU/ml (2.0 McFarland density) prepared from a 3-day blood agar plate0.5 to 1 × 109 CFU/ml (4.0 McFarland density) prepared from a 2-day blood agar plate
Incubation35°C; microaerobic atmosphere (gas system- generated microaerobic atmosphere)37°C; microaerobic atmosphere
Reading3 days3 days
Quality control strain(s)H. pylori ATCC 43504H. pylori CCUG 38770, H. pylori CCUG 38771, H. pylori CCUG 38772
Quality control limitsAmoxicillin, ciprofloxacin, clarithromycin, metronidazole, tetracyclineAmoxicillin, clarithromycin, metronidazole

From: Chapter 42, Antibiotic Susceptibility and Resistance

Cover of Helicobacter pylori
Helicobacter pylori: Physiology and Genetics.
Mobley HLT, Mendz GL, Hazell SL, editors.
Washington (DC): ASM Press; 2001.
Copyright © 2001, ASM Press.

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