Figure 1. Vaccination with H.

Figure 1

Vaccination with H. pylori. Groups of C57BL/6 wild-type donor mice were immunized intranasally with 100 μg H. pylori lysate antigen and 10 μg CT adjuvant, or with CT adjuvant alone. H. pylori immunization protected from infection relative to treatment with CT alone (2.9 × 103 vs. 2.4 × 105 CFU/biopsy, respectively; p < 0.0001 by Wilcoxon rank sum analysis). Spleen Th-cell effectors were isolated from protected donor mice and expanded in vitro with H. pylori lysate, IL-2, and IL-4. H. pylori-specific CD4+T cells were then magnetically sorted and adoptively transferred (106) by intravenous injection into groups of C57BL/6, MHC class II−/−, or CD4−/− recipient mice. The mice were subsequently challenged with H. pylori, and immune protection was assessed 2 weeks later by quantitative bacterial culture. Micrographs show representative cross sections (7 μm) of stomach from the indicated strains. T cells were stained with anti-TCRβ and anti-CD4, and the mean frequency per mm3 of stomach was derived from serial sections. The circles enclose TCRαβ+CD4+ cells. The H. pylori infection density represents the mean CFU/biopsy × 105. Protection represents the extent of attenuation of the infection in adoptively transferred mice compared with untreated mice, analyzed by Wilcoxon rank sum test. Adapted from reference 87.

From: Chapter 37, Vaccines

Cover of Helicobacter pylori
Helicobacter pylori: Physiology and Genetics.
Mobley HLT, Mendz GL, Hazell SL, editors.
Washington (DC): ASM Press; 2001.
Copyright © 2001, ASM Press.

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