NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.

Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002.

  • By agreement with the publisher, this book is accessible by the search feature, but cannot be browsed.
Cover of Biochemistry

Biochemistry. 5th edition.

Show details

Section 13.6Gap Junctions Allow Ions and Small Molecules to Flow between Communicating Cells

The ion channels that we have considered thus far have narrow pores and are moderately to highly selective in regard to which ions are permeant. They are closed in the resting state and have short lifetimes in the open state, typically a millisecond, that enable them to transmit highly frequent neural signals. We turn now to a channel with a very different role. Gap junctions, also known as cell-to-cell channels, serve as passageways between the interiors of contiguous cells. Gap junctions are clustered in discrete regions of the plasma membranes of apposed cells. Electron micrographs of sheets of gap junctions show them tightly packed in a regular hexagonal array (Figure 13.30A). A 20-Å central hole, the lumen of the channel, is prominent in each gap junction. A tangential view (Figure 13.30B) shows that these channels span the intervening space, or gap, between apposed cells (hence, the name gap junction). The width of the gap between the cytosols of the two cells is about 35 Å.

Figure 13.30. Gap Junctions.

Figure 13.30

Gap Junctions. (A) This electron micrograph shows a sheet of isolated gap junctions. The cylindrical connexons form a hexagonal lattice having a unit-cell length of 85 Å. The densely stained central hole has a diameter of about 20 Å. (B) (more...)

Small hydrophilic molecules as well as ions can pass through gap junctions. The pore size of the junctions was determined by microinjecting a series of fluorescent molecules into cells and observing their passage into adjoining cells. All polar molecules with a mass of less than about 1 kd can readily pass through these cell-to-cell channels. Thus, inorganic ions and most metabolites (e.g., sugars, amino acids, and nucleotides) can flow between the interiors of cells joined by gap junctions. In contrast, proteins, nucleic acids, and polysaccharides are too large to traverse these channels. Gap junctions are important for intercellular communication. Cells in some excitable tissues, such as heart muscle, are coupled by the rapid flow of ions through these junctions, which ensure a rapid and synchronous response to stimuli. Gap junctions are also essential for the nourishment of cells that are distant from blood vessels, as in lens and bone. Moreover, communicating channels are important in development and differentiation. For example, a pregnant uterus is transformed from a quiescent protector of the fetus to a forceful ejector at the onset of labor; the formation of functional gap junctions at that time creates a syncytium of muscle cells that contract in synchrony.

A cell-to-cell channel is made of 12 molecules of connexin, one of a family of transmembrane proteins with molecular masses ranging from 30 to 42 kd. Each connexin molecule appears to have four membrane-spanning helices. Six connexin molecules are hexagonally arrayed to form a half channel, called a connexon or hemichannel (Figure 13.31). Two connexons join end to end in the intercellular space to form a functional channel between the communicating cells. Cell-to-cell channels differ from other membrane channels in three respects: (1) they traverse two membranes rather than one; (2) they connect cytosol to cytosol, rather than to the extracellular space or the lumen of an organelle; and (3) the connexons forming a channel are synthesized by different cells. Gap junctions form readily when cells are brought together. A cell-to-cell channel, once formed, tends to stay open for seconds to minutes. They are closed by high concentrations of calcium ion and by low pH. The closing of gap junctions by Ca2+ and H+ serves to seal normal cells from traumatized or dying neighbors. Gap junctions are also controlled by membrane potential and by hormone-induced phosphorylation.

Figure 13.31. Schematic Representation of a Gap Junction.

Figure 13.31

Schematic Representation of a Gap Junction. [Courtesy of Dr. Werner Loewenstein.]

By agreement with the publisher, this book is accessible by the search feature, but cannot be browsed.

Copyright © 2002, W. H. Freeman and Company.
Bookshelf ID: NBK22492


  • Cite this Page
  • Disable Glossary Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...