Table 8.5KM values of some enzymes

EnzymeSubstrateKM(μM)
ChymotrypsinAcetyl-l-tryptophanamide5000
LysozymeHexa-N-acetylglucosamine6
β-GalactosidaseLactose4000
Threonine deaminaseThreonine5000
Carbonic anhydraseCO28000
PenicillinaseBenzylpenicillin50
Pyruvate carboxylasePyruvate400
HCO3-1000
ATP60
Arginine-tRNA synthetaseArginine3
tRNA0.4
ATP300

From: Section 8.4, The Michaelis-Menten Model Accounts for the Kinetic Properties of Many Enzymes

Cover of Biochemistry
Biochemistry. 5th edition.
Berg JM, Tymoczko JL, Stryer L.
New York: W H Freeman; 2002.
Copyright © 2002, W. H. Freeman and Company.

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