Figure 4.13. Electrophoretic Analysis of a Protein Purification.

Figure 4.13Electrophoretic Analysis of a Protein Purification

The purification scheme in Table 4.1 was analyzed by SDS-PAGE. Each lane contained 50 μg of sample. The effectiveness of the purification can be seen as the band for the protein of interest becomes more prominent relative to other bands.

From: Section 4.1, The Purification of Proteins Is an Essential First Step in Understanding Their Function

Cover of Biochemistry
Biochemistry. 5th edition.
Berg JM, Tymoczko JL, Stryer L.
New York: W H Freeman; 2002.
Copyright © 2002, W. H. Freeman and Company.

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