Figure 8-40. (a) The absorption of ultraviolet light of 260-nm wavelength by solutions of single-stranded and double-stranded DNA.

Figure 8-40

(a) The absorption of ultraviolet light of 260-nm wavelength by solutions of single-stranded and double-stranded DNA. As regions of double-stranded DNA unpair, the absorption of light by those regions increases almost twofold. The temperature at which half the bases in a double-stranded DNA sample have denatured is denoted T m. (b) DNA melting curves. The absorbance relative to that at 25°C is plotted against temperature. (The wavelength of the incident light was 260 nm.) The T m is 69°C for E. coli DNA (50 percent GC pairs) and 76°C for Pseudomonas aeruginosa DNA (68 percent GC pairs). (Part b from L. Stryer, Biochemistry, 4th ed. Copyright © 1995 by Lubert Stryer.)

From: Mechanism of DNA replication

Cover of An Introduction to Genetic Analysis
An Introduction to Genetic Analysis. 7th edition.
Griffiths AJF, Miller JH, Suzuki DT, et al.
New York: W. H. Freeman; 2000.
Copyright © 2000, W. H. Freeman and Company.

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