TABLE 2Analysis of Selenium

ReagentSe DetectedResult of ReactionDetection Limit (µg/ml)Interfering Substances
ThioureaSe+4pink color or red ppt.5Te, NO2-, Cu, Hg, Bi, Au, Pt, Pd
Hydroxylamine HClSe+4pink color or red ppt.5many elements except Te
IodideSe+4red-brown ppt.40As+3, Ge +4, Mo+6
Thiocyanic acidSe+4red-brown ppt.2As, Sb, Sn, Fe+2, MoO 4-2
PyrroleSe+4pyrrole blue color0.5oxidizing elements, Se+6, Te+4, Te +6
Asymmetric diphenyl-hydrazineSe+4red color2oxidizing agents
Methylene blue and NaS2Se0decolorization3oxidizing agents
Ammonium molybdateSe+4molybdenum-selenium blue color3PO4-3, SO4-2
3,3 ′- diamino-benzidineSe+4yellow color or red fluorescence0.01oxidizing agents, Fe+3, Cu +2
2,3- diamino-naphthaleneSe+4yellow color or green fluorescence0.002oxidizing agents

SOURCE: Nazarenko and Ermakov (1972)

From: 2, Chemistry

Cover of Selenium in Nutrition
Selenium in Nutrition: Revised Edition.
National Research Council (US) Subcommittee on Selenium.
Washington (DC): National Academies Press (US); 1983.
Copyright © National Academy of Sciences.

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