Figure 7-7. Ligation of restriction fragments with complementary sticky ends.

Figure 7-7Ligation of restriction fragments with complementary sticky ends

In this example, EcoRI fragments from DNA I (left) are mixed with several different restriction fragments, including EcoRI fragments, produced from DNA II (right). The short DNA sequences composing the sticky ends of each fragment type are shown. The complementary sticky ends on the two types of EcoRI fragments, (a′) and (a), can transiently base-pair, whereas the TaqI fragments (b) and HindIII fragments (c) with noncomplementary sticky ends do not base-pair to EcoRI fragments. The adjacent 3′-hydroxyl and 5′-phosphate groups (red) on the base-paired fragments then are covalently joined (ligated) by T4 DNA ligase. One ATP is consumed for each phosphodiester bond (red) formed.

From: Section 7.1, DNA Cloning with Plasmid Vectors

Cover of Molecular Cell Biology
Molecular Cell Biology. 4th edition.
Lodish H, Berk A, Zipursky SL, et al.
New York: W. H. Freeman; 2000.
Copyright © 2000, W. H. Freeman and Company.

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