NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.

Berg JM, Tymoczko JL, Stryer L. Biochemistry. 5th edition. New York: W H Freeman; 2002.

  • By agreement with the publisher, this book is accessible by the search feature, but cannot be browsed.
Cover of Biochemistry

Biochemistry. 5th edition.

Show details

Preface

For more than 25 years, and through four editions, Stryer’s Biochemistry has laid out this beautiful subject in an exceptionally appealing and lucid manner. The engaging writing style and attractive design have made the text a pleasure for our students to read and study throughout our years of teaching. Thus, we were delighted to be given the opportunity to participate in the revision of this book. The task has been exciting and somewhat daunting, doubly so because of the dramatic changes that are transforming the field of biochemistry as we move into the twenty-first century. Biochemistry is rapidly progressing from a science performed almost entirely at the laboratory bench to one that may be explored through computers. The recently developed ability to determine entire genomic sequences has provided the data needed to accomplish massive comparisons of derived protein sequences, the results of which may be used to formulate and test hypotheses about biochemical function. The power of these new methods is explained by the impact of evolution: many molecules and biochemical pathways have been generated by duplicating and modifying existing ones. Our challenge in writing the fifth edition of Biochemistry has been to introduce this philosophical shift in biochemistry while maintaining the clear and inviting style that has distinguished the preceding four editions.Figure 9.44

A New Molecular Evolutionary Perspective

How should these evolution-based insights affect the teaching of biochemistry? Often macromolecules with a common evolutionary origin play diverse biological roles yet have many structural and mechanistic features in common. An example is a protein family containing macromolecules that are crucial to moving muscle, to transmitting the information that adrenaline is present in the bloodstream, and to driving the formation of chains of amino acids. The key features of such a protein family, presented to the student once in detail, become a model that the student can apply each time that a new member of the family is encountered. The student is then able to focus on how these features, observed in a new context, have been adapted to support other biochemical processes. Throughout the text, a stylized tree icon Image tree.jpg is positioned at the start of discussions focused primarily on protein homologies and evolutionary origins.

Two New Chapters

To enable students to grasp the power of these insights, two completely new chapters have been added. The first, “Biochemical Evolution” (Chapter 2), is a brief tour from the origin of life to the development of multicellular organisms. On one level, this chapter provides an introduction to biochemical molecules and pathways and their cellular context. On another level, it attempts to deepen student understanding by examining how these molecules and pathways arose in response to key biological challenges. In addition, the evolutionary perspective of Chapter 2 makes some apparently peculiar aspects of biochemistry more reasonable to students. For example, the presence of ribonucleotide fragments in biochemical cofactors can be accounted for by the likely occurrence of an early world based largely on RNA. The second new chapter, “Exploring Evolution” (Chapter 7), develops the conceptual basis for the comparison of protein and nucleic acid sequences. This chapter parallels “Exploring Proteins” (Chapter 4) and “Exploring Genes” (Chapter 6), which have thoughtfully examined experimental techniques in earlier editions. Its goal is to enable students to use the vast information available in sequence and structural databases in a critical and effective manner.

Organization of the Text

The evolutionary approach influences the organization of the text, which is divided into four major parts. As it did in the preceding edition, Part I introduces the language of biochemistry and the structures of the most important classes of biological molecules. The remaining three parts correspond to three major evolutionary challenges—namely, the interconversion of different forms of energy, molecular reproduction, and the adaptation of cells and organisms to changing environments. This arrangement parallels the evolutionary path outlined in Chapter 2 and naturally flows from the simple to the more complex.

PART I, the molecular design of life, introduces the most important classes of biological macromolecules, including proteins, nucleic acids, carbohydrates, and lipids, and presents the basic concepts of catalysis and enzyme action. Here are two examples of how an evolutionary perspective has shaped the material in these chapters:

  • Chapter 9, on catalytic strategies, examines four classes of enzymes that have evolved to meet specific challenges: promoting a fundamentally slow chemical reaction, maximizing the absolute rate of a reaction, catalyzing a reaction at one site but not at many alternative sites, and preventing a deleterious side reaction. In each case, the text considers the role of evolution in fine-tuning the key property.
  • Chapter 13, on membrane channels and pumps, includes the first detailed three-dimensional structures of an ion channel and an ion pump. Because most other important channels and pumps are evolutionarily related to these proteins, these two structures provide powerful frameworks for examining the molecular basis of the action of these classes of molecules, so important for the functioning of the nervous and other systems.
    PART II, transducing and storing energy, examines pathways for the interconversion of different forms of energy. Chapter 15, on signal transduction, looks at how DNA fragments encoding relatively simple protein modules, rather than entire proteins, have been mixed and matched in the course of evolution to generate the wiring that defines signal-transduction pathways. The bulk of Part II discusses pathways for the generation of ATP and other energy-storing molecules. These pathways have been organized into groups that share common enzymes. The component reactions can be examined once and their use in different biological contexts illustrated while these reactions are fresh in the students’ minds.
  • Chapter 16 covers both glycolysis and gluconeogenesis. These pathways are, in some ways, the reverse of each other, and a core of enzymes common to both pathways catalyze many of the steps in the center of the pathways. Covering the pathways together makes it easy to illustrate how free energy enters to drive the overall process either in the direction of glucose degradation or in the direction of glucose synthesis.
  • Chapter 17, on the citric acid cycle, ties together through evolutionary insights the pyruvate dehydrogenase complex, which feeds molecules into the citric acid cycle, and the α-ketoglutarate dehydrogenase complex, which catalyzes one of the key steps in the cycle itself.Figure 15.34
  • Oxidative phosphorylation, in Chapter 18, is immediately followed in Chapter 19 by the light reactions of photosynthesis to emphasize the many common chemical features of these pathways.
  • The discussion of the light reactions of photosynthesis in Chapter 19 leads naturally into a discussion of the dark reactions—that is, the components of the Calvin cycle—in Chapter 20. This pathway is naturally linked to the pentose phosphate pathway, also covered in Chapter 20, because in both pathways common enzymes interconvert three-, four-, five-, six-, and seven-carbon sugars.

PART III, synthesizing the molecules of life, focuses on the synthesis of biological macromolecules and their components.

  • Chapter 24, on the biosynthesis of amino acids, is linked to the preceding chapter on amino acid degradation by a family of enzymes that transfer amino groups to and from the carbon frameworks of amino acids.
  • Chapter 25 covers the biosynthesis of nucleotides, including the role of amino acids as biosynthetic precursors. A key evolutionary insight emphasized here is that many of the enzymes in these pathways are members of the same family and catalyze analogous chemical reactions. The focus on enzymes and reactions common to these biosynthetic pathways allows students to understand the logic of the pathways, rather than having to memorize a set of seemingly unrelated reactions.
  • Chapters 27, 28, and 29 cover DNA replication, recombination, and repair; RNA synthesis and splicing; and protein synthesis. Evolutionary connections between prokaryotic systems and eukaryotic systems reveal how the basic biochemical processes have been adapted to function in more-complex biological systems. The recently elucidated structure of the ribosome gives students a glimpse into a possible early RNA world, in which nucleic acids, rather than proteins, played almost all the major roles in catalyzing important pathways.

PART IV, responding to environmental changes, looks at how cells sense and adapt to changes in their environments. Part IV examines, in turn, sensory systems, the immune system, and molecular motors and the cytoskeleton. These chapters illustrate how signaling and response processes, introduced earlier in the text, are integrated in multicellular organisms to generate powerful biochemical systems for detecting and responding to environmental changes. Again, the adaptation of proteins to new roles is key to these discussions.

Integrated Chemical Concepts

We have attempted to integrate chemical concepts throughout the text. They include the mechanistic basis for the action of selected enzymes, the thermodynamic basis for the folding and assembly of proteins and other macromolecules, and the structures and chemical reactivity of the common cofactors. These fundamental topics underlie our understanding of all biological processes. Our goal is not to provide an encyclopedic examination of enzyme reaction mechanisms. Instead, we have selected for examination at a more detailed chemical level specific topics that will enable students to understand how the chemical features help meet the biological needs.

Chemical insight often depends on a clear understanding of the structures of biochemical molecules. We have taken considerable care in preparing stereochemically accurate depictions of these molecules where appropriate. These structures should make it easier for the student to develop an intuitive feel for the shapes of molecules and comprehension of how these shapes affect reactivity.

Image fmfu1.jpg

Newly Updated to Include Recent Discoveries

Given the breathtaking pace of modern biochemistry, it is not surprising that there have been major developments since the publication of the fourth edition. Foremost among them is the sequencing of the human genome and the genomes of many simpler organisms. The text’s evolutionary framework allows us to naturally incorporate information from these historic efforts. The determination of the three-dimensional structures of proteins and macromolecular assemblies also has been occurring at an astounding pace.

  • As noted earlier, the discussion of excitable membranes in Chapter 13 incorporates the detailed structures of an ion channel (the prokaryotic potassium channel) and an ion pump (the sacroplasmic reticulum calcium ATPase).Figure 9.21
  • Great excitement has been generated in the signal transduction field by the first determination of the structure of a seven-transmembrane-helix receptor—the visual system protein rhodopsin—discussed in Chapters 15and 32
  • The ability to describe the processes of oxidative phosphorylation in Chapter 18 has been greatly aided by the determination of the structures for two large membrane protein complexes: cytochrome c oxidase and cytochrome bc1.
  • Recent discoveries regarding the three-dimensional structure of ATP synthase are covered in Chapter 18, including the remarkable fact that parts of the enzyme rotate in the course of catalysis.
  • The determination of the structure of the ribosome transforms the discussion of protein synthesis in Chapter 29.
  • The elucidation of the structure of the nucleosome core particle—a large protein–DNA complex— facilitates the description in Chapter 31 of key processes in eukaryotic gene regulation.

Finally, each of the three chapters in Part IV is based on recent structural conquests.

  • The ability to grasp key concepts in sensory systems (Chapter 32) is aided by the structures of rhodopsin and the aforementioned ion channel.
  • Chapter 33, on the immune system, now includes the more recently determined structure of the T-cell receptor and its complexes.
  • The determination of the structures of the molecular motor proteins myosin and kinesin first revealed the evolutionary connections on which Chapter 34, on molecular motors, is based.

New and Improved Illustrations

The relation of structure and function has always been a dominant theme of Biochemistry. This relation becomes even clearer to students using the fifth edition through the extensive use of molecular models. These models are superior to those in the fourth edition in several ways.

  • All have been designed and rendered by one of us (JMB), with the use of MOLSCRIPT, to emphasize the most important structural features. The philosophy of the authors is that the reader should be able to write the caption from looking at the picture.
  • We have chosen ribbon diagrams as the most effective, clearest method of conveying molecular structure. All molecular diagrams are rendered in a consistent style. Thus students are able to compare structures easily and to develop familiarity and facility in interpreting the models. Labels highlight key features of the molecular models.
  • Many new molecular models have been added, serving as sources of structural insight into additional molecules and in some cases affording multiple views of the same molecule.

In addition to the molecular models, the fifth edition includes more diagrams providing an overview of pathways and processes and setting processes in their biological context.

Image fmfu2.jpg

New Pedagogical Features

The fifth edition of Biochemistry supplies additional tools to assist students in learning the subject matter.

Icons

Icons are used to highlight three categories of material, making these topics easier to locate for the interested student or teacher.

  • Image caduceus.jpgA caduceus signals the beginning of a clinical application.
  • Image tree.jpgA stylized tree marks sections or paragraphs that primarily or exclusively explore evolutionary aspects of biochemistry.
  • Image mouse.jpg A mouse and finger point to references to animations on the text’s Web site (www.whfreeman.com/biochem5) for those students who wish to reinforce their understanding of concepts by using the electronic media.

More Problems

The number of problems has increased by 50%. Four new categories of problem have been created to develop specific skills.

  • Mechanism problems ask students to suggest or elaborate a chemical mechanism.
  • Data interpretation problems ask questions about a set of data provided in tabulated or graphic form. These exercises give students a sense of how scientific conclusions are reached.
  • Chapter integration problems require students to use information from multiple chapters to reach a solution. These problems reinforce awareness of the interconnectedness of the different aspects of biochemistry.
  • Media problems encourage and assist students in taking advantage of the animations and tutorials provided on our Web site. Media problems are found both in the book and on our Web site.Figure 15.23

New Chapter Outline and Key Terms

An outline at the beginning of each chapter gives major headings and serves as a framework for students to use in organizing the information in the chapter. The major headings appear again in the chapter’s summary, again helping to organize information for easier review. A set of key terms also helps students focus on and review the important concepts.Figure 17.4

Tools and Techniques

The fifth edition of Biochemistry offers three chapters that present the tools and techniques of biochemistry: “Exploring Proteins” (Chapter 4), “Exploring Genes” (Chapter 6), and “Exploring Evolution” (Chapter 7). Additional experimental techniques are presented elsewhere throughout the text, as appropriate.

Exploring Proteins (Chapter 4)

Protein purification  Section 4.1

Differential centrifugation  Section 4.1.2

Salting out  Section 4.1.3

Dialysis  Section 4.1.3

Gel-filtration chromatography  Section 4.1.3

Ion-exchange chromatography  Section 4.1.3

Affinity chromatography   Section 4.1.3

High-pressure liquid chromatography  Section 4.1.3

Gel electrophoresis  Section 4.1.4

Isoelectric focusing  Section 4.1.4

Two-dimensional electrophoresis  Section 4.1.4

Qualitative and quantitative evaluation of protein purification  Section 4.1.5

Ultracentrifugation  Section 4.1.6

Mass spectrometry (MALDI-TOF)  Section 4.1.7

Peptide mass fingerprinting  Section 4.1.7

Edman degradation  Section 4.2

Protein sequencing  Section 4.2

Production of polyclonal antibodies  Section 4.3.1

Production of monoclonal antibodies  Section 4.3.2

Enzyme-linked immunosorbent assay (ELISA)   Section 4.3.3

Western blotting  Section 4.3.4

Fluorescence microscopy  Section 4.3.5

Green fluorescent protein as a marker  Section 4.3.5

Immunoelectron microscopy  Section 4.3.5

Automated solid-phase peptide synthesis  Section 4.4

Nuclear magnetic resonance spectroscopy  Section 4.5.1

NOESY spectroscopy  Section 4.5.1

X-ray crystallography  Section 4.5.2

Exploring Proteins (other chapters)

Basis of fluorescence in green fluorescent protein   Section 3.6.5

Time-resolved crystallography  Section 8.3.2

Using fluorescence spectroscopy to analyze enzyme– substrate interactions  Section 8.3.2

Using irreversible inhibitors to map the active site   Section 8.5.2

Using transition state analogs to study enzyme active sites  Section 8.5.3

Catalytic antibodies as enzymes  Section 8.5.4

Exploring Genes (Chapter 6)

Restriction-enzyme analysis  Sections 6.1.1 and 6.1.2

Southern and Northern blotting techniques  Section 6.1.2

Sanger dideoxy method of DNA sequencing   Section 6.1.3

Solid-phase analysis of nucleic acids  Section 6.1.4

Polymerase chain reaction (PCR)  Section 6.1.5

Recombinant DNA technology  Sections 6.2-6.4

DNA cloning in bacteria  Sections 6.2.2 and 6.2.3

Chromosome walking  Section 6.2.4

Cloning of eukaryotic genes in bacteria  Section 6.3.1

Examining expression levels (gene chips)  Section 6.3.2

Introducing genes into eukaryotes  Section 6.3.3

Transgenic animals  Section 6.3.4

Gene disruption  Section 6.3.5

Tumor-inducing plasmids  Section 6.3.6

Site-specific mutagenesis  Section 6.4

Exploring Genes (other chapters)

Density-gradient equilibrium sedimentation   Section 5.2.2

Footprinting technique for isolating and characterizing promoter sites  Section 28.1.1

Chromatin immunoprecipitation (ChIP)  Section 31.2.3

Exploring Evolution (Chapter 7)

Sequence-comparison methods  Section 7.2

Sequence-alignment methods  Section 7.2

Estimating the statistical significance of alignments (by   shuffling)  Section 7.2.1

Substitution matrices  Section 7.2.2

Sequence templates  Section 7.3.2

Self-diagonal plots for finding repeated motifs  Section 7.3.3

Mapping secondary structures through RNA sequence   comparisons  Section 7.3.5

Construction of evolutionary trees  Section 7.4

Combinatorial chemistry  Section 7.5.2

Other Techniques

Sequencing of carbohydrates by using MALDI-TOF   mass spectrometry  Section 11.3.7

Use of liposomes to investigate membrane permeability     Section 12.4.1

Use of hydropathy plots to locate transmembrane   helices  Section 12.5.4

Fluorescence recovery after photobleaching (FRAP)   for measuring lateral diffusion in membranes     Section 12.6

Patch-clamp technique for measuring channel activity     Section 13.5.1

Measurement of redox potential  Section 18.2.1

Functional magnetic resonance imaging (fMRI)     Section 32.1.3

Image mouse.jpg Animated Techniques: Animated explanations of experimental techniques used for exploring genes and proteins are available at www.whfreeman.com/biochem5

Clinical Applications

Image caduceus.jpg This icon signals the start of a clinical application in the text. Additional, briefer clinical correlations appear without the icon in the text as appropriate.

Prion diseases  Section 3.6.1

Scurvy and collagen stabilization  Section 3.6.5

Antigen detection with ELISA  Section 4.3.3

Vasopressin deficiency  Section 4.4

Action of penicillin  Section 8.5.5

Water-soluble vitamins  Section 8.6.1

Fat-soluble vitamins in blood clotting and vision  Section 8.6.2

Protease inhibitors  Section 9.1.7

Carbonic anhydrase and osteopetrosis  Section 9.2

Use of isozymes to diagnose tissue damage  Section 10.3

Emphysema  Section 10.5.4

Thromboses prevention  Section 10.5.7

Hemophilia  Section 10.5.8

Regulation of blood clotting  Section 10.5.9

Blood groups  Section 11.2.5

Antibiotic inhibitors of glycosylation  Section 11.3.3

I-cell disease  Section 11.3.5

Selectins and the inflammatory response  Section 11.4.1

Influenza virus  Section 11.4.2

Clinical uses of liposomes  Section 12.4.1

Aspirin and ibuprofen  Section 12.5.2

Digitalis and congestive heart failure  Section 13.2.3

Multidrug resistance and cystic fibrosis  Section 13.3

Protein kinase inhibitors as anticancer drugs  Section 15.5.1

Cholera and whooping cough  Section 15.5.2

Lactose intolerance  Section 16.1.12

Galactose toxicity  Section 16.1.13

Cancer and glycolysis  Section 16.2.5

Phosphatase deficiency and lactic acidosis  Section 17.2.1

Beriberi and poisoning by mercury and arsenic  Section 17.3.2

Mitochondrial diseases  Section 18.6.5

Hemolytic anemia  Section 20.5.1

Glucose 6-phosphate dehydrogenase deficiency   Section 20.5.2

Glycogen-storage diseases  Section 21.5.4

Steatorrhea in liver disease  Section 22.1.1

Carnitine deficiency  Section 22.2.3

Zellweger syndrome  Section 22.3.4

Diabetic ketosis  Section 22.3.6

Use of fatty acid synthase inhibitors as drugs  Section 22.4.9

Effects of aspirin on signaling pathways  Section 22.6.2

Cervical cancer and ubiquitin  Section 23.2.1

Protein degradation and the immune response  Section 23.2.3

Inherited defects of the urea cycle (hyperammonemia)   Section 23.4.4

Inborn errors of amino acid degradation  Section 23.6

High homocysteine levels and vascular disease  Section 24.2.9

Inherited disorders of porphyrin metabolism  Section 24.4.4

Anticancer drugs that block the synthesis of thymidylate  Section 25.3.3

Pellagra  Section 25.5

Gout  Section 25.6.1

Lesch-Nyhan syndrome  Section 25.6.2

Disruption of lipid metabolism as the cause of respiratory distress syndrome and Tay-Sachs disease  Section 26.1.6

Diagnostic use of blood cholesterol levels  Section 26.3.2

Hypercholesteremia and atherosclerosis  Section 26.3.5

Clinical management of cholesterol levels  Section 26.3.6

Rickets and vitamin D  Section 26.4.7

Antibiotics that target DNA gyrase  Section 27.3.4

Defective repair of DNA and cancer  Section 27.6.5

Huntington chorea  Section 27.6.6

Detection of carcinogens (Ames test)  Section 27.6.7

Antibiotic inhibitors of transcription  Section 28.1.9

Burkitt lymphoma and B-cell leukemia  Section 28.2.6

Thalassemia  Section 28.3.3

Antibiotics that inhibit protein synthesis  Section 29.5.1

Diphtheria  Section 29.5.2

Prolonged starvation  Section 30.3.1

Diabetes  Section 30.3.2

Regulating body weight  Section 30.3.3

Metabolic effects of ethanol  Section 30.5

Anabolic steroids  Section 31.3.3

SERMs and breast cancer  Section 31.3.3

Color blindness  Section 32.3.5

Use of capsaicin in pain management  Section 32.5.1

Immune system suppressants  Section 33.4.3

MHC and transplantation rejection  Section 33.5.6

AIDS vaccine  Section 33.5.7

Autoimmune diseases  Section 33.6.2

Immune system and cancer  Section 33.6.3

Myosins and deafness  Section 34.2.1

Kinesins and nervous system disorders  Section 34.3

Taxol  Section 34.3.1

Molecular Evolution

Image tree.jpg This icon signals the start of many discussions that highlight protein commonalities or other molecular evolutionary insights that provide a framework to help students organize information.

Why this set of 20 amino acids?  Section 3.1

Many exons encode protein domains  Section 5.6.2

Catalytic triads in hydrolytic enzymes  Section 9.1.4

Major classes of peptide-cleaving enzymes  Section 9.1.6

Zinc-based active sites in carbonic anhydrases   Section 9.2.4

A common catalytic core in type II restriction enzymes   Section 9.3.4

P-loop NTPase domains  Section 9.4.4

Fetal hemoglobin  Section 10.2.3

A common catalytic core in protein kinases  Section 10.4.3

Why might human blood types differ?  Section 11.2.5

Evolutionarily related ion pumps  Section 13.2

P-type ATPases  Section 13.2.2

ATP-binding cassette domains  Section 13.3

Secondary transporter families  Section 13.4

Acetylcholine receptor subunits  Section 13.5.2

Sequence comparisons of sodium channel cDNAs   Section 13.5.4

Potassium and sodium channel homologies  Section 13.5.5

Using sequence comparisons to understand sodium and calcium channels  Section 13.5.7

Evolution of metabolic pathways  Section 14.3.4

How Rous sarcoma virus acquired its oncogene  Section 15.5

Recurring features of signal-transduction pathways   Section 15.6

Why is glucose a prominent fuel?  Section 16.0.1

A common binding site in dehydrogenases  Section 16.1.10

The major facilitator (MF) superfamily of transporters   Section 16.2.4

Isozymic forms of lactate dehydrogenase  Section 16.4.2

Evolutionary relationship of glycolysis and gluconeogenesis Section 16.4.3

Decarboxylation of α-ketoglutarate and pyruvate   Section 17.1.6

Evolution of succinyl CoA synthetase  Section 17.1.7

Evolutionary history of the citric acid cycle  Section 17.3.3

Endosymbiotic origins of mitochondria  Section 18.1.2

Conservation of cytochrome c structure  Section 18.3.7

Common features of ATP synthase and G proteins   Section 18.4.5

Related uncoupling proteins  Section 18.6.4

Evolution of chloroplasts  Section 19.1.2

Evolutionary origins of photosynthesis  Section 19.6

Evolution of the C4 pathway  Section 20.2.3

Increasing sophistication of glycogen phosphorylase regulation  Section 21.3.3

The α-amylase family  Section 21.4.3

A recurring motif in the activation of carboxyl groups   Section 22.2.2

Polyketide and nonribosomal peptide synthetases resemble fatty acid synthase  Section 22.4.10

Prokaryotic counterparts of the ubiquitin pathway and the proteasome  Section 23.2.4

A family of pyridoxal-dependent enzymes  Section 23.3.3

Evolution of the urea cycle  Section 23.4.3

The P-loop NTPase domain in nitrogenase  Section 24.1.1

Recurring steps in purine ring synthesis  Section 25.2.3

Ribonucleotide reductases  Section 25.3

Increase in urate levels during primate evolution  Section 25.6.1

The cytochrome P450 superfamily  Section 26.4.3

DNA polymerases  Section 27.2.1

Helicases  Section 27.2.5

Evolutionary relationship of recombinases and topoisomerases  Section 27.5.2

Similarities in transcriptional machinery between archaea and eukaryotes  Section 28.2.4

Evolution of spliceosome-catalyzed splicing  Section 28.2.4

Classes of aminoacyl-tRNA synthetases  Section 29.2.5

Composition of the primordal ribosome  Section 29.3.1

Evolution of molecular mimics  Section 29.4.4

A family of proteins with common ligand-binding domains   Section 31.1.4

Independent evolution of DNA-binding sites of regulatory proteins  Section 31.1.5

CpG islands  Section 31.2.5

Iron response elements  Section 31.4.2

The odorant receptor family  Section 32.1.1

Evolution of taste receptor mRNA  Section 32.2.5

Photoreceptor evolution  Section 32.3.4

The immunoglobulin fold  Section 33.2

Relationship of actin to hexokinase and other prokaryotic proteins  Section 34.2.2

Tubulins in the P-loop NTPase family  Section 34.3.1

Supplements Supporting Biochemistry, Fifth Edition

The fifth edition of Biochemistry offers a wide selection of high-quality supplements to assist students and instructors.

For the Instructor

Print and Computerized Test Banks NEW

Marilee Benore Parsons, University of Michigan-Dearborn  Print Test Bank  0-7167-4384-1; Computerized Test Bank CD-ROM (Windows/Macintosh hybrid)  0-7167-4386-8

The test bank offers more than 1700 questions posed in multiple choice, matching, and short-answer formats. The electronic version of the test bank allows instructors to easily edit and rearrange the questions or add their own material.

Instructor’s Resource CD-ROM NEW

© W. H. Freeman and Company and Sumanas, Inc.  0-7167-4385-X

The Instructor’s Resource CD-ROM contains all the illustrations from the text. An easy-to-use presentation manager application, Presentation Manager Pro, is provided. Each image is stored in a variety of formats and resolutions, from simple jpg and gif files to preformatted PowerPoint® slides, for instructors using other presentation programs.

Overhead Transparencies

0-7167-4422-8

Full-color illustrations from the text, optimized for classroom projection, in one volume.

For the Student

Student Companion

Richard I. Gumport, College of Medicine at Urbana-Champaign, University of Illinois; Frank H. Deis, Rutgers University; and Nancy Counts Gerber, San Fransisco State University. Expanded solutions to text problems provided by Roger E. Koeppe II, University of Arkansas 0-7167-4383-3

Image fmfu3.jpg

More than just a study guide, the Student Companion is an essential learning resource designed to meet the needs of students at all levels. Each chapter starts with a summarized abstract of the related textbook chapter. A comprehensive list of learning objectives allows students to quickly review the key concepts. A self-test feature allows students to quickly refresh their understanding, and a set of additional problems requires students to apply their knowledge of biochemistry. The complete solution to every problem in the text is provided to help students better comprehend the core ideas. Individual chapters of the Student Companion can be purchased and downloaded from

www.whfreeman.com/biochem5

Clinical Companion NEW

Kirstie Saltsman, Ph.D., Jeremy M. Berg, M.D., and Gordon Tomaselli, M.D., Johns Hopkins University School of Medicine 0-7167-4738-3

Image fmfu4.jpg

Designed for students and instructors interested in clinical applications, the Clinical Companion is a rich compendium of medical case studies and clinical discussions. It contains numerous problems and references to the textbook. Such topics as glaucoma, cystic fibrosis, Tay-Sachs disease, and autoimmune diseases are covered from a biochemical perspective.

Lecture Notebook NEW

0-7167-4682-4

For students who find that they are too busy writing notes to pay attention in class, the Lecture Notebook brings together a black-and-white collection of illustrations from the text, arranged in the order of their appearance in the textbook, with plenty of room alongside for students to take notes.

Experimental Biochemistry, Third Edition

Robert L. Switzer, University of Illinois, and Liam F. Garrity, Pierce Chemical Corporation  0-7167-3300-5

The new edition of Experimental Biochemistry has been completely revised and updated to make it a perfect fit for today’s laboratory course in biochemistry. It provides comprehensive coverage of important techniques used in contemporary biochemical research and gives students the background theory that they need to understand the experiments. Thoroughly classroom tested, the experiments incorporate the full range of biochemical materials in an attempt to simulate work in a research laboratory. In addition, a comprehensive appendix provides detailed procedures for preparation of reagents and materials, as well as helpful suggestions for the instructor.

Image fmfu5.jpg

Also Available Through the W. H. Freeman Custom Publishing Program

Experimental Biochemistry is designed to meet all your biochemistry laboratory needs. Visit http://custompub.whfreeman.com to learn more about creating your own laboratory manual.

Student Media Resources

Image mouse.jpg This icon links materials from the book to our Web site. See the inside front cover for a complete description of the resources available at www.whfreeman.com/ biochem5.

Image fmfu6.jpg
W. H. Freeman and Company is proud to present a weekly collection of abstracts and a monthly featured article from the Nature family of journals, including Nature, its associated monthly titles, and the recently launched Nature Review Journals. Please visit www.whfreeman.com/biochem5 for more information.

By agreement with the publisher, this book is accessible by the search feature, but cannot be browsed.

Copyright © 2002, W. H. Freeman and Company.
Bookshelf ID: NBK21159