Genomes fuses the fresh outlook of the new genomics with the traditional approach to gene expression to provide an up-to-date understanding of the role of the genome as the blueprint for life. This integrated approach focuses on the topics that are central to molecular genetics to create a teaching resource for modern molecular biology.

Contents

• Preface
• An Introduction to Genomes
• Part 1. Genomes, Transcriptomes and Proteomes
  • Chapter 1. The Human Genome
    • 1.1 DNA
    • 1.2 The Human Genome
    • 1.3 Why is the Human Genome Project Important?
    • Study Aids For Chapter 1
    • References
    • Further Reading
  • Chapter 2. Genome Anatomies
    • 2.1 An Overview of Genome Anatomies
    • 2.2 The Anatomy of the Eukaryotic Genome
    • 2.3 The Anatomy of the Prokaryotic Genome
    • 2.4 The Repetitive DNA Content of Genomes
    • Study Aids For Chapter 2
    • References
    • Further Reading
  • Chapter 3. Transcriptomes and Proteomes
    • 3.1 Genome Expression in Outline
    • 3.2 The RNA Content of the Cell
    • 3.3 The Protein Content of the Cell
    • Study Aids For Chapter 3
    • References
    • Further Reading
• Part 2. Studying Genomes
  • Chapter 4. Studying DNA
    • 4.1 Enzymes for DNA Manipulation
    • 4.2 DNA Cloning
    • 4.3 The Polymerase Chain Reaction (PCR)
    • Study Aids For Chapter 4
    • References
    • Further Reading
  • Chapter 5. Mapping Genomes
    • 5.1 Genetic and Physical Maps
    • 5.2 Genetic Mapping
    • 5.3 Physical Mapping
    • Study Aids For Chapter 5
    • References
    • Further Reading
  • Chapter 6. Sequencing Genomes
    • 6.1 The Methodology for DNA Sequencing
    • 6.2 Assembly of a Contiguous DNA Sequence
    • 6.3 The Human Genome Projects
    • Study Aids For Chapter 6
    • References
    • Further Reading
  • Chapter 7. Understanding a Genome Sequence
    • 7.1 Locating the Genes in a Genome Sequence
    • 7.2 Determining the Functions of Individual Genes
    • 7.3 Global Studies of Genome Activity
    • 7.4 Comparative Genomics
    • Study Aids For Chapter 7
    • References
    • Further Reading
• Part 3. How Genomes Function
  • Chapter 8. Accessing the Genome
    • 8.1 Inside the Nucleus
    • 8.2 Chromatin Modifications and Genome Expression
    • Study Aids For Chapter 8
    • References
    • Further Reading
  • Chapter 9. Assembly of the Transcription Initiation Complex
    • 9.1 The Importance of DNA-binding Proteins
    • 9.2 DNA-Protein Interactions During Transcription Initiation
    • 9.3 Regulation of Transcription Initiation
    • Study Aids For Chapter 9
    • References
    • Further Reading
  • Chapter 10. Synthesis and Processing of RNA
    • 10.1 Synthesis and Processing of mRNA
    • 10.2 Synthesis and Processing of Non-coding RNAs
    • 10.3 Processing of Pre-RNA by Chemical Modification
    • 10.4 Degradation of mRNAs
    • 10.5 Transport of RNA Within the Eukaryotic Cell
    • Study Aids For Chapter 10
    • References
    • Further Reading
  • Chapter 11. Synthesis and Processing of the Proteome
    • 11.1 The Role of tRNA in Protein Synthesis
    • 11.2 The Role of the Ribosome in Protein Synthesis
    • 11.3 Post-translational Processing of Proteins
    • 11.4 Protein Degradation
    • Study Aids For Chapter 11
    • References
    • Further Reading
  • Chapter 12. Regulation of Genome Activity
    • 12.1 Transient Changes in Genome Activity
    • 12.2 Permanent and Semipermanent Changes in Genome Activity
    • 12.3 Regulation of Genome Activity During Development
    • Study Aids For Chapter 12
    • References
    • Further Reading
• Part 4. How Genomes Replicate and Evolve
  • Chapter 13. Genome Replication
    • 13.1 The Topological Problem
    • 13.2 The Replication Process
    • 13.3 Regulation of Eukaryotic Genome Replication
    • Study Aids For Chapter 13
    • References
    • Further Reading
  • Chapter 14. Mutation, Repair and Recombination
    • 14.1 Mutations
    • 14.2 DNA Repair
    • 14.3 Recombination
    • Study Aids For Chapter 14
    • References
    • Further Reading
  • Chapter 15. How Genomes Evolve
    • 15.1 Genomes: the First 10 Billion Years
    • 15.2 Acquisition of New Genes
    • 15.3 Non-coding DNA and Genome Evolution
    • 15.4 The Human Genome: the Last 5 Million Years
    • Study Aids For Chapter 15
    • References
    • Further Reading
  • Chapter 16. Molecular Phylogenetics
    • 16.1 The Origins of Molecular Phylogenetics
    • 16.2 The Reconstruction of DNA-based Phylogenetic Trees
    • 16.3 The Applications of Molecular Phylogenetics
    • Study Aids For Chapter 16
    • References
    • Further Reading
• Appendix
• Glossary
• Abbreviations