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Brown TA. Genomes. 2nd edition. Oxford: Wiley-Liss; 2002.

Cover of Genomes

Genomes. 2nd edition.

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Part 2Studying Genomes

Virtually everything we know about genomes and genome expression has been discovered by scientific research; theoretical studies have played very little role in this or any other area of molecular and cell biology. It is possible to learn ‘facts’ about genomes without knowing very much about how those facts were obtained, but in order to gain a real understanding of the subject we must first examine in detail the techniques and scientific approaches that have been used to study genomes. Armed with this technical background, you will be able to tackle the remainder of the book with the confidence that comes from a thorough appreciation of how molecular biology research is carried out.

Part 2 of Genomes takes a staged approach to genetic and genomic research methods. First, in Chapter 4, we examine the techniques, centered on the polymerase chain reaction and DNA cloning, that are used to study DNA molecules. These techniques are very effective with short segments of DNA, including individual genes, enabling a wealth of information to be obtained at this level.

Chapter 5 moves on to the methods developed to construct maps of genomes, and describes how the techniques of genetic mapping, first developed almost a century ago, have been supplemented with complementary methods for direct physical mapping of genomes.

Chapter 6 makes the link between mapping and sequencing, and shows that although a map can be a valuable aid to assembly of a long DNA sequence, mapping is not always a prerequisite to genome sequencing.

Finally, in Chapter 7, we look at the various approaches that are used to understand a genome sequence. As you read this chapter you will begin to appreciate that locating the genes in a genome sequence, and identifying their functions, is one of the major research challenges of modern biology.

Chapter 4. Studying DNA

Chapter 5. Mapping Genomes

Chapter 6. Sequencing Genomes

Chapter 7. Understanding a Genome Sequence

Copyright © 2002, Garland Science.
Bookshelf ID: NBK21119

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