| Number of Plasmids with Base Changes† | ||
|---|---|---|
| Mutations | XP | Normal |
| Independent plasmids sequenced‡ | 61 (100%) | 89 (100%) |
| Point mutations | ||
| Single base substitution | 47§ (77%) | 48 (53%) |
| Tandem base substitutions|| | 12 (20%) | 16 (18%) |
| Multiple base substitutions# | 1§ (2%) | 24 (28%) |
| Base insertions and deletions | ||
| Single base insertion | 0 | 2 |
| Single or tandem base deletions | 1 | 3 |
| Types of single or tandem base substitutions and number of changes | ||
| XP | Normal | |
| Transitions | 67§ (94%) | 61 (75%) |
| GC to AT | 66§ (93%) | 59 (73%) |
| AT to GC | 1 (1%) | 2 (2%) |
| Transversions | 4§ (6%) | 20 (25%) |
| GC to TA | 0§ | 8 (10%) |
| GC to CG | 1 (1%) | 5 (6%) |
| AT to TA | 3 (4%) | 6 (8%) |
| AT to CG | 0 | 1 (1%) |
Modified from Bredberg et al.112
50 to 300 J/m2 for XP cells, 100 to 5,000 J/m2 for normal cells.
From separate transfections or different mutations in the same transfection including all experiments.
p < .01 versus normal.
Two base substitutions 0 to 2 bases apart, or 3 adjacent base substitutions.
At least 2 base substitutions more than 3 bases apart.
From: Chapter 15, Ultraviolet Radiation Carcinogenesis
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