FIGURE 13.9. 2D optical spectroscopy (2DOS).


2D optical spectroscopy (2DOS). Images are collected simultaneously at four wavelengths (560 nm, 570 nm, 577 nm, 610 nm) using a four-way beam splitter. Images are spatially registered, and grayscale changes are normalized. Each registered pixel thus has four grayscale values (one for each wavelength) which change over time. For each pixel at each time point, least squares analysis is used to fit the measured absorbance changes to changes in oxy- and deoxyhemoglobin concentration using a modified form of the Beer–Lambert law. Oxy- and deoxyhemoglobin absorption coefficients for each wavelength are derived from an in vitro phantom, thus accounting for wavelength-dependent path differences in the brain.

From: Chapter 13, Intraoperative Optical Imaging

Cover of In Vivo Optical Imaging of Brain Function
In Vivo Optical Imaging of Brain Function. 2nd edition.
Frostig RD, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2009.
Copyright © 2009, Taylor & Francis Group, LLC.

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