FIGURE 18.1. (A) O-Linked β-N-acetylglucosamine (O-GlcNAc) is a dynamic protein modification abundant within the nucleus and cytoplasm.

FIGURE 18.1

(A) O-Linked β-N-acetylglucosamine (O-GlcNAc) is a dynamic protein modification abundant within the nucleus and cytoplasm. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues. O-GlcNAcase hydrolyzes O-GlcNAc from proteins. The two enzymes often occur within the same complex and are therefore highly regulated in controlling O-GlcNAc cycling. (B) Two-dimensional (2D) gel analysis of immunoaffinity-isolated O-GlcNAc–modified proteins from nucleocytoplasmic extracts of HeLa cells. The 2D gel analysis reveals that many proteins are modified by O-GlcNAc.

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From: Chapter 18, The O-GlcNAc Modification

Cover of Essentials of Glycobiology
Essentials of Glycobiology. 2nd edition.
Varki A, Cummings RD, Esko JD, et al., editors.
Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009.
Copyright © 2009, The Consortium of Glycobiology Editors, La Jolla, California.

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