FIGURE 27.6. Preparation of covalent glycan microarrays printed on N-hydroxysuccinimide (NHS)- or epoxide-activated glass slides.

FIGURE 27.6

Preparation of covalent glycan microarrays printed on N-hydroxysuccinimide (NHS)- or epoxide-activated glass slides. In this example, the glycans have a free amine at the reducing end and are coupled to the glass slide. After washing, the slide is “interrogated” with a GBP. After washing away the unbound GBP, binding is detected by fluorescence. The GBP may be directly fluorescently labeled or detected using fluorescently labeled secondary reagents. The slides are read in a fluorescence scanner.

Symbol Key: FIGURE 27.6. Preparation of covalent glycan microarrays printed on N-hydroxysuccinimide (NHS)- or epoxide-activated glass slides.

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From: Chapter 27, Principles of Glycan Recognition

Cover of Essentials of Glycobiology
Essentials of Glycobiology. 2nd edition.
Varki A, Cummings RD, Esko JD, et al., editors.
Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009.
Copyright © 2009, The Consortium of Glycobiology Editors, La Jolla, California.

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