Comparative Genomics is a collection of robust protocols for molecular biologists beginning to use comparative genomic analysis tools in a variety of areas. The three chapters that are available on the Bookshelf describe National Center for Biotechnology Information (NCBI) resources available to the public, two of which are based on the NCBI Mini-Courses. These chapters appear in Volumes 1 and 2 of Comparative Genomics, which comprise Volumes 395 and 396 of the Methods in Molecular Biology series.

Contents

• 9. BLAST QuickStart: Example-Driven Web-Based BLAST Tutorial
  David Wheeler and Medha Bhagwat.
  • 1 Introduction
  • 2 Course and Website
  • 3 Nucleotide BLAST
  • 4 Protein BLAST
  • 5 Translated BLAST
  • 6 Genome BLAST
  • 7 BLAST2Sequences
  • 7.2 Examples of Two Sequence Comparisons
  • 8 Notes
  • References
• 10. PSI-BLAST Tutorial
  Medha Bhagwat and L. Aravind.
  • 1 Introduction
  • 2 Problem 1
  • 3 Problem 2
  • 4 Caveats to Remember While Using PSI-BLAST
  • 5 Notes
  • References
• 24. Identification of Disease Genes: Example-Driven Web-Based Tutorial
  Medha Bhagwat.
  • 1 Introduction to the National Center for Biotechnology Information Mini-Courses
  • 2 Objective
  • 3 Genetics and Bioinformatics Background
  • 4 General Protocol and Required Resources
  • 5 Detailed Protocol: Problem 1
  • 6 Detailed Protocol: Problem 2
  • 7 Application to Unknown Disease Genes
  • 8 Further Analysis
  • 9 Notes
  • References

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