Table 5Isolated thioflavine S and primuline components for NS3 helicase inhibitor discovery

Compound IDcompound nametarget potency, MBHA (IC50, avg, μM)cell-based assaysantitarget potency (IC50, avg, μM)
#CID*1b(con1)replicon (% inhibition @ 10 μM)cell viability (% @ 10 μM)FID (EtBr)ATPase
SID2a(JFH1)FID (SYBR Green I)Protease
1415676Pthioflavine S24± 1.3−16 ± −283 ± 2nd>200
16 ± 561 ± 37nd
2415713Pprimuline12 ± 1.31098 ± 373 ± 36nd
9.6 ± 1.143 ± 14nd
344251429ST133 ± 24099 ± 2>100>200
8528542725 ± 21.8>100nd
444251431ST24.6 ± 1.6096 ± 7>100>200
852854284.7 ± 0.626 ± 4nd
544251427SP170 ± 310104 ± 4>100>200
8528542521 ± 7.7>200nd
644251428SP25.6 ± 1.6
45 ± 14
95 ± 14
9 ± 2
55 ± 8
112 ± 4
95 ± 4
85 ± 7
105 ± 5
>100
>100
nd
>200
>200
>200
85285426
104178066
126920288
7.3 ± 2.2
58 ± 50.7
nd
23 ± 4
>100
>100
nd
nd
nd
744251436SP30.9 ± 0.0259 ± 1091 ± 655 ± 20nd
852854310.6 ± 0.3ndnd
844251437
44251438
SP40.8 ± 0.2
2.2 ± 0.1
0
45 ± 14
94 ± 3
83 ± 8
15 ± 3
76 ± 1
nd
43 ± 10
85285432
99438075
0.5 ± 0.4
1.2 ± 0.3
nd
14 ± 3
nd
2 ± 1
944251433SP1a>100088 ± 7>100>200
8528542948.5 ± 54.4>100nd
1044251434SP2a7 ± 3.828 ± 587 ± 7>100>200
852854303.9 ± 0.432 ± 3nd
*

Denotes whether the compound was synthesized (S) or purchased (P).

Please refer to Figure 8 for compound structures. All the isolated compounds are ammonium salts except P4.

From: Hepatitis C Virus NS3 Helicase Inhibitor Discovery

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.