Table 2Typical protocol of primary and secondary assays

StepParameterValueDescription
1Reagent2 μLEnzyme
2Compound23 nL0.5 nM to 58 μM final concentrations (in titration)
3Reagent2 μLSubstrate
4Time40 min37 °C incubation
5Reagent2 μLStop solution (1 M NaOH and 1 M Glycine mixture, pH 10)
6DetectorLuminescenceViewLux plate reader

Concentrations of enzyme and substrate varied, and exact information can be found in PubChem. Please see appendix for list of PubChem AIDs.

From: Discovery, SAR, and Biological Evaluation of Non-inhibitory Chaperones of Glucocerebrosidase

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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