Figure 5. Competitive ABPP with clickable N-heterocyclic urea activity-based probes 6–10 (AA6–AA10) to assess general SH reactivity.

Figure 5Competitive ABPP with clickable N-heterocyclic urea activity-based probes 610 (AA6–AA10) to assess general SH reactivity

A. Competitive ABPP of compounds 610 (AA6–AA10) in the mouse brain membrane proteome with FP-Rh. Control = DMSO only, no compound. B. Profiling the direct targets of 810 (AA8–AA10, 20 μM) in brain membranes in the presence or absence of the SH-directed probe FP-biotin (20 μM) followed by click chemistry to append an azide-Rh tag under click chemistry conditions. Targets of compound 10 (AA10) that are not competed by FP-biotin (presumably non-SH proteins) are highlighted with red boxes. (fluorescent images shown in grey scale)

From: Optimization and characterization of a triazole urea inhibitor for platelet-activating factor acetylhydrolase type 2 (PAFAH2)

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