Figure 4.. Schematic of teratoma formation.

Figure 4.

Schematic of teratoma formation. An overview of teratoma formation: culture hPSCs until the desired amount is achieved, harvest the cells using scrapers or Collagenase IV, pellet cells, add equal volume of liquid Matrigel to the cell mixture (make sure to keep cells on ice), transplant mixture into mouse models, allow mice to recover on heat pad, image mice regularly, and explant teratomas for analysis.

From: Teratoma formation: A tool for monitoring pluripotency in stem cell research

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StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2012 Wendy Y. Zhang, Patricia E. de Almeida, and Joseph C. Wu.

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