Figure 2. Basic quality control of iPSC colonies.

Figure 2Basic quality control of iPSC colonies.

Selecting “good” colonies from “bad” ones is probably the most commonly used quality control step in hPSC research. Here we show iPSCs generated from fibroblasts with episomal vectors and stages of propagating them until the cells have lost the episomes at about passage 15 (Okita, Matsumura et al., 2011). Only colonies with “good” morphology (flat appearance, well defined edges, homogenous cell morphology) are selected in each passage and carried forward.

From: Assessment of human pluripotent stem cells with PluriTest

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StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2012 Franz-Josef Müller, Björn Brändl and Jeanne F. Loring.

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