Figure 1: . Location of sixteen positive controls (red) and sixteen negative controls (yellow) on a 384-well plate.

Figure 1:

Location of sixteen positive controls (red) and sixteen negative controls (yellow) on a 384-well plate. In layout (A), both sets of controls are located on the plate edges in a regular pattern, and are susceptible to edge-based bias. In contrast, layouts (B) and (C) attempt to systematically decrease the edge bias by alternating the spatial position of the controls so that they appear in equal quantity on each of the rows and available columns. Adapted from (1).

From: Advanced Assay Development Guidelines for Image-Based High Content Screening and Analysis

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
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