Figure 4: . Fluorescence-based Ion Flux Assay Formats.

Figure 4:

Fluorescence-based Ion Flux Assay Formats. Shown from left to right are examples of fluorescence-based ion flux assays. Plasma membrane calcium permeable channels (BLUE) and endoplasmic reticular (ER) calcium permeable channels (PURPLE) can be measured with a variety of fluorescent calcium indicator dyes like Fluo-2 and Fura-2 (not shown). Sodium permeable channels (RED) can be measured by sodium sensitive fluorescent dyes. Potassium channels (GREEN) can be assayed using a surrogate ion approach and the thallium sensitive dye, FluoZin-2. Unlike the other fluorescent dyes mentioned above, the mutant yellow fluorescent protein (YFP)-based sensor has its fluorescence effectively quenched by the surrogate ion, iodide, fluxing through a chloride channel (YELLOW).

From: Ion Channel Screening

Cover of Assay Guidance Manual
Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
Copyright Notice

All Assay Guidance Manual content, except where otherwise noted, is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported license (CC BY-NC-SA 3.0), which permits copying, distribution, transmission, and adaptation of the work, provided the original work is properly cited and not used for commercial purposes. Any altered, transformed, or adapted form of the work may only be distributed under the same or similar license to this one.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.