Figure 22: . Freeze-thaw cycle stability of reference compound inhibitors IK101 and IK202.

Figure 22:

Freeze-thaw cycle stability of reference compound inhibitors IK101 and IK202. Reference inhibitor compounds were made at 10mM in DMSO and stored at –80oC, and then allowed to thaw at room temperature before use. Compound vial samples were then re-frozen at –80oC multiple times following thaw. HeLa cells seeded at 5,000 cells/well overnight and treated in dose response with reference compound inhibitors IK101 (left) or IK202 (right) for 15 minutes followed by stimulation with 25ηg/ml TNF-α for ~35 minutes. Cells were then fixed and stained to measure NF-κB translocation. Plates were analyzed on HCS imager to determine NF-κB translocation using CytoNuc Difference calculation; data was normalized to control and plotted in GraphPad Prism using non-linear regression 3-parameter fit.

From: Nuclear Factor Kappa B (NF-κB) Translocation Assay Development and Validation for High Content Screening

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Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
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