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Items: 4

1.

alignment of 3' and 5' splice sites of mRNA

Recognition of both the 5' and 3'-splice sites and positioning them in the correct alignment with respect to each other so that the second catalytic step of nuclear mRNA splicing can occur. Synonym: alignment of 3' and 5' splice sites of nuclear mRNA;

Type
:
pathway
Taxonomic scope
:
conserved biosystem
BSID:
503605
GO
: GO:0034403
2.

pre-mRNA 5'-splice site binding

Interacting selectively and non-covalently with the pre-mRNA 5' splice site sequence. Synonym: pre-mRNA 5' splice site binding;

Type
:
functional_set
Taxonomic scope
:
conserved biosystem
BSID:
499935
GO
: GO:0030627
3.

second spliceosomal transesterification activity

Catalysis of the second transesterification reaction of spliceosomal mRNA splicing. Ligation of the two exons occurs via a transesterification reaction where the free 3'-hydroxyl group of the 5' exon is the nucleophile attacking the 3' splice site. Non-expressed sequences are now...

Type
:
functional_set
Taxonomic scope
:
conserved biosystem
BSID:
486871
GO
: GO:0000386
4.

generation of catalytic spliceosome for second transesterification step

Conformational rearrangement of the spliceosomal complex containing the RNA products from the 1st step of splicing to form the catalytic site for the second step of splicing. Synonyms: GO:0000358; GO:0000359; catalytic spliceosome assembly for second transesterification step; formation...

Type
:
pathway
Taxonomic scope
:
conserved biosystem
BSID:
486866
GO
: GO:0000350

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