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UI-CF-EC1

Identifiers
BioSample: SAMN00170276; EST: LIBEST_010395
Organism
Homo sapiens (human)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Primates; Haplorrhini; Simiiformes; Catarrhini; Hominoidea; Hominidae; Homininae; Homo
Attributes
development stageAdult and Fetal
tissueLung
lab hostDH10B (Life Technologies) (T1 phage resistant)
tissueLung
re_1EcoR I
v_typePlasmid
re_2Not I
vectorpT7T3-Pac (Pharmacia) with a modified polylinker
Description

UI-CF-EC1 is a normalized cDNA library containing the following tissue(s): Normal lung from adult and from fetal day 64, day 87, week 19 and week 42. The library was constructed according to Bonaldo, Lennon and Soares, Genome Research, 6:791-806, 1996. First strand cDNA synthesis was primed with an oligo-dT primer containing a Not I site. Double stranded cDNA was ligated to an EcoR I adaptor, digested with Not I, and cloned directionally into pT7T3-Pac vector. The oligonucleotide used to prime the synthesis of first-strand cDNA contains a library tag sequence that is located between the Not I site and the (dT)18 tail. The sequence tag for this library is AAGTGCTTAC.

Submission
University of Iowa, McCray, PB; 2002-03-21
Accession:
SAMN00170276
ID:
170276

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