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Poly A RNA was prepared from total RNA prep. Full-size, double-stranded cDNA was prepared by SMART synthesis and normalized using kamchatka crab duplex-specific nuclease. SfiI-digested cDNA was cloned in SfiIA and SfiIB sites in pAL17.3 plasmid vector. Sequencing was carried out on a template consisting of vector DNA from cDNA-carrying clones selected by blue/white screeing.
Nucleotide
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