Composite genome of "Caldiarchaeum subterraneus"

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Identifiers: BioSample: SAMD00016619; SRA: DRS000201

Organism: microbial mat metagenome
unclassified entries; unclassified sequences; metagenomes; ecological metagenomes

Attributes

sample name	DRS000201
sample comment	This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine. As a first step of this project, we constructed a fosmid library from the DNA directly isolated from the microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we determined the paired-end sequence of all fosmid clones. We selected putative archaeal genome fragments from the metagenomic library. Total of 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. In addition, 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences.

sample name

DRS000201

sample comment

This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine. As a first step of this project, we constructed a fosmid library from the DNA directly isolated from the microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we determined the paired-end sequence of all fosmid clones. We selected putative archaeal genome fragments from the metagenomic library. Total of 132 fosmids were grouped into each 13 or 20 fosmid and each group was analyzed in a half plate of 454 GS20. In addition, 18 fosmids were analyzed by Sanger sequencing. A composite archaeal genome was reconstructed from 91 complete or partial fosmid sequences.

BioProjects: PRJDA49157 Candidatus Caldarchaeum subterraneum
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PRJNA39577 microbial mat metagenome
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Submission: Genome Analysis Research Group, Extremophiles Research Program, Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology; 2010-04-14