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Mannheimia haemolytica USDA-ARS-184 Phase Variant 20240815.SB

Identifiers
BioSample: SAMN43497902; Sample name: M. haemolytica phase variant Mh184.20240815.PV.SB
Organism
Mannheimia haemolytica
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Mannheimia
Package
MIGS: cultured bacteria/archaea; version 6.0
Attributes
strainMh184.20240815.PV.SB
collection date2024-08-15
broad-scale environmental contextENVO:00002123
local-scale environmental contextENVO:00002124
environmental mediumMICRO:0000067
geographic locationUSA: Clay Center, NE
isolation and growth condition5955799
latitude and longitudeNA
number of replicons1
reference for biomaterialNA
collection method chemically defined minimal medium aerobic culture of SMOOTH colony Sample ID 209472
encoded traitsSMOOTH phenotype
estimated size2500000
hostBos taurus
host diseaseBovine Respiratory Disease Complex
host taxonomy ID9913 (9913)
isolation sourceDerived from an aerobic bioreactor culture in chemically defined minimal medium (CDMM) inoculated with a suspension of a single SMOOTH colony (Sample ID 209472) grown on blood agar base + hemin plate (BABH) medium. This BABH colony was isolated from a glycerol stock of SMOOTH colonies with Sample ID 66060, a child of Sample ID 184, BioSample SAMN03275447.
pathogenicityBos taurus
relationship to oxygenfacultative
source material identifiers209472
subspecific genetic lineagephase variant
ancestor_biosampleSAMN03275447
Description

A glycerol stock of SMOOTH colonies (Sample ID 66060, a child of Sample ID 184, BioSample SAMN03275447 ) was plated on blood agar base + hemin (BABH) medium and grown overnight at 37C under 5% CO2 to generate SMOOTH colonies. A heavy streak of these SMOOTH colonies was struck out again on BABH yielding consistently SMOOTH colonies on BABH. A single colony (source material Sample ID 209472) on the BABH plate was selected for aerobic culture in chemically defined minimal medium (CDMM) in a 50 ml conical with 15 mL CDMM for 21 hours; 191 rpm 37C. Caps lightly cracked open. DNA was extracted from this culture for PacBio HiFi sequencing.

Keywords: GSC:MIxS;MIGS:6.0

BioProject
PRJNA172854 Mannheimia haemolytica
Retrieve all samples from this project

Submission
USDA-ARS-USMARC, Gregory Harhay; 2024-09-04
Accession:
SAMN43497902
ID:
43497902

BioProject Nucleotide

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