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spindrop mouse old RNA 5EU-seq sample 2

Identifiers
BioSample: SAMN36871499; SRA: SRS18529510; GEO: GSM7688450
Organism
Mus musculus (house mouse)
cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Attributes
source nameEmbryos at E8.5 whole embryo
tissueEmbryos at E8.5 whole embryo
cell lineCD-1
cell typeembryos
treatmentSample was dissected and cultured for 3 hours in IVC1 with the 5EU analog, cells were then dissociated with TrypLE Express for 15 minutes at 37C, Click-chemistry was performed as per the scEU-seq protocol and cells were processed using spindrop without sorting. After de-emulsification and clean-up the "old" samples are collected in the supernatant from the streptavidin pull-down before downstream library preparation.
geographic locationmissing
collection datemissing
Links
GEO Sample GSM7688450
BioProject
PRJNA858488 spinDrop: a modular droplet microfluidic platform to maximise single-cell RNA sequencing information content
Retrieve all samples from this project

Submission
University of Cambridge, Florian Hollfelder; 2023-08-07
Accession:
SAMN36871499
ID:
36871499

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