BioSample

Phylum-specific changes in bacterial abundance and diversity in marine environments are due to several factors, including seasonal temperature cycles. To identify species which predominate during winter, we harvested bacteria from intertidal sediment (water temperature = 7 degrees C, March 2019) offshore of Virginia Beach, VA. Sediment and pore water from a depth of 1 meter were placed into a sterile plastic jar. Sediment was diluted (1:10) in Phosphate Buffered Saline (PBS), pH 7.4, vortexed, spread on Zobell Marine Agar 2216 plates, and incubated for five days at 4 degrees C. Isolated colonies were picked, purified, and maintained at 4 degrees C.