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Cannabis sativa 'Finola' genomic DNA

Identifiers
BioSample: SAMN00738506; Sample name: CS-FINOLA-DNA; SRA: SRS266713
Organism
Cannabis sativa (hemp)
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Cannabaceae; Cannabis
Package
Plant; version 1.0
Attributes
cultivarFinola
tissueyoung leaf
agemissing
development stagemissing
geographic locationmissing
Description

Genomic DNA was purified from nuclei isolated from young leaves. Briefly, leaves were frozen in liquid nitrogen, extracted with homogenization buffer (10 mM Tris pH 9.5, 80 mM KCl, 10 mM EDTA, 1 mM spermidine, 1 mM spermine, 0.15% β-mercaptoethanol and 0.5% Triton X-100) with gentle stirring and the extract filtered through cheesecloth and Miracloth. The nuclei and cell debris were pelleted by centrifugation and resuspended in wash buffer (as above with 0.5% (v/v) Triton X-100 and 0.15% β-mercaptoethanol) using a paintbrush. After low-speed centrifugation to remove cell debris, the nuclei were recovered by centrifugation. The nuclei were resuspended in wash buffer and centrifuged three times to remove other organelles. Genomic DNA was isolated from purified nuclei by treatment with proteinase K, extraction with phenol-chloroform and precipitation with isopropanol. The precipitated DNA was spooled using a glass hook, washed with 70% ethanol and treated with RNase. The DNA was extracted with phenol-chloroform, precipitated with sodium acetate/ethanol, spooled with a glass hook, washed with 70% ethanol and air dried. The purified DNA was dissolved in TE buffer.

BioProject
PRJNA73819 Cannabis sativa
Retrieve all samples from this project

Submission
University of Toronto, Harm Van Bakel; 2011-10-11
Accession:
SAMN00738506
ID:
738506

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