BioSample

Protocols: The culture was shock-cooled after 30 min of incubation in presence of the inducer. 2 x 2 ml culture were centrifuged per replicate. The cell pellet was then frozen at -80 degrees for a maximum of one week. Cells were grown in Lennox LB broth to an OD of 0.5, then the inducer was added (0.2% rhamnose final concentration). Incubation then continued for 30 min prior to harvest. The RiboPure bacteria kit from Invitrogen was used for RNA extraction, using the manufacturer's protocol. 200 ng of RNA was depleted for rRNA using the Ribo-Zero Magnetic Kit for Gram-negative Bacteria (Epicentre). Library preparation was performed using the Truseq stranded Total RNA library prep (Illumina) according to manufacturer's protocol. Libraries were quantified by qPCR.