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Accession: PRJNA94037 ID: 94037

Saccharomyces cerevisiae (baker's yeast)

Short term perturbation

See Genome Information for Saccharomyces cerevisiae
Study of the short term (within the first 330 seconds) transcriptional response of S.cerevisiae upon a sudden addition of glucose. Keywords: glucose pulse, chemostat culture, glucose catabolite repression Overall design: Chemostat cultivation – Saccharomyces cerevisiae (CEN PK 113-7D) was cultivated in an aerobic carbon-limited chemostat culture in a 7-litres fermentor (Applikon, The Netherlands) with a working volume of 4-l on the adapted doubled mineral medium (Verduyn et al., 1992) with 27.1 g.l-1 of glucose and 1.42 g.l-1 of ethanol, to support a biomass concentration of about 15 g dry weight.l-1. The dilution rate was set to be 0.05 hr-1 and the airflow rate was set to be 200 l.hr-1. Other fermentation parameters are: a pH controlled at 5, a temperature controlled at 30˚C, an overpressure of 0.3 bar and stirrer speed of 600 rpm. The chemostat was considered to obtain its stable steady state condition 5 resident times after the end of its batch phase. Glucose pulse experiment - At the age of 7 dilution times, the steady state chemostat culture was perturbed by the addition of 20 ml of glucose solution (200 g/l) to the fermentor so that the residual glucose concentration was suddenly increased to about 1 g/l (5.56 mM). The glucose solution was rapidly injected by a pneumatic system (< 1 s). Samples were taken prior to the glucose pulse (steady state samples) and within 360 s transient after the perturbation. Verduyn,C., Postma,E., Scheffers,W.A., and van Dijken,J.P. (1992). Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8, 501-517.
AccessionPRJNA94037; GEO: GSE3821
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismSaccharomyces cerevisiae[Taxonomy ID: 4932]
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces cerevisiae
PublicationsKresnowati MT et al., "When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation.", Mol Syst Biol, 2006 Sep 12;2:49
SubmissionRegistration date: 14-Dec-2005
Kluyver centre for genomics of industrial organisms, Department of Biotechnology, Delft University of Technology
RelevanceModel Organism
Project Data:
Resource NameNumber
of Links
Publications
PubMed1
PMC1
Other datasets
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Spots149360
Data volume, Processed Mbytes4
Data volume, Supplementary Mbytes29

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