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Genome Information for Homo sapiens
Acute aerobic exercise has been shown to improve skeletal muscle mitochondrial function and completeness of fatty acid β-oxidation which contribute to improved insulin sensitivity. The effectiveness of acute exercise on improving mitochondrial adaptations, leading to improved insulin sensitivity, in overweight/obese (Ov/Ob) individuals is controversial. This study aimed to determine the effects of acute exercise on epigenetic regulation, specifically nucleosome positioning, of genes involved in skeletal muscle mitochondrial adaptations in lean vs Ov/Ob men.
Overall design: 30 men, aged 19-30 years, were recruited and divided into two groups: lean (BMI<25, 18.5- 24.1 kg/m2, n=15) and Ov/Ob (BMI≥25, 25.5- 36.9 kg/m2, n=15). Vastus lateralis muscle biopsy samples were collected at rest, following an overnight fast (Pre; n=14 in lean Pre and n=15 in Ov/Ob Pre). Subjects then ingested a standardized high-carbohydrate breakfast (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein). Four hours later, they performed an acute bout of cycling exercise (50% VO2max, expending ~650 kcal) and vastus lateralis muscle biopsy samples were collected immediately after the exercise bout (Post; n=15 in lean Post and in Ov/Ob Post). Mononucleosomal DNA was extracted from vastus lateralis muscle samples (n=5 per group) and MNase-seq was performed using the Illumina platform and analyzed via HOMER and custom scripts, as previously described (Henagan, TM et al 2015).
Accession | PRJNA427931; GEO: GSE108642 |
Data Type | Epigenomics |
Scope | Multiisolate |
Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
Publications | Devarshi PP et al., "A single bout of cycling exercise induces nucleosome repositioning in the skeletal muscle of lean and overweight/obese individuals.", Diabetes Obes Metab, 2022 Jan;24(1):21-33 |
Submission | Registration date: 29-Dec-2017 Medical School, Louisiana State University Health Sciences Center - Shreveport |
Relevance | Medical |
Project Data:
Resource Name | Number of Links |
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Sequence data |
SRA Experiments | 4 |
Publications |
PubMed | 1 |
PMC | 1 |
Other datasets |
BioSample | 4 |
GEO DataSets | 1 |