BioProject

For this bio project, we profiled entire ribosomal operons from bacteria within our complex samples. More...

For this bio project, we profiled entire ribosomal operons from bacteria within our complex samples. These rRNA operons are amplified using domain-specific forward primers in the small ribosomal RNA subunit (16S), domain-specific reverse primers within the large rRNA subunit (23S), and a High Fidelity Taq polymerase. This rRNA operon profiling approach yields an amplicon containing both 16S/23S ribosomal subunits for phylogenetic assignment (4200 bp of sequence), plus the ITS region (500+ bp) containing species/strain information to distinguish various members within the microbial community. Less...