BioProject

The goal of this study is to obtain comprehensive gene expression profiling data for the main extracellular developmental stages of the human pathogen Trypanosoma cruzi using an RNA-seq approach. More...

The goal of this study is to obtain comprehensive gene expression profiling data for the main extracellular developmental stages of the human pathogen Trypanosoma cruzi using an RNA-seq approach. An array of computational tools was applied to map reads to the T. cruzi genome, reconstruct full-length transcripts and determine mRNA abundance enabling us to identify co-expression patterns that correlate with the biology of the parasite. We have defined trans-splicing and polyadenylation sites and identified novel T. cruzi genes that were likely missed during the original annotation, thereby greatly enhancing current gene model structures and annotations. The study provides a solid framework for future functional and genomic studies of Chagas disease as well as intracellular pathogenesis in general. Less...