무료 슬롯 게임 2-온라인 카지노 1000$ 무료【eggcsite.com】iKQ - BioProject

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Select item 9993201.

Expression data from leaves of tomato (Solanum lycopersicum L. cv. MicroTom) plants subjected to 1000 Hz sound vibration (at 90 dB for 2 h for 3 consecutive days) and/or inoculated with Pst DC300.

Organism:: Solanum lycopersicum

Taxonomy:: Solanum lycopersicum (tomato)

: Project data type: Transcriptome or Gene expression

Scope:: Multiisolate

Universidad Politécnica de Cartagena

Accession:: PRJNA999320
ID:: 999320

Select item 7634522.

Dissecting iNKT cell selection and subset differentiation in real-time

To systematically investigate the earliest phases of iNKT cell development and subsequent subset differentiation, we generated a genetic system to induce a timed wave of iNKT cell generation upon 4-hydroxytamoxifen (4-OHT) administration in mice bearing CD4-CreERt2 (Sledzinska et al., 2013) and Vα14iStopF (Vahl et al., 2013) knock-in transgenes. We globally investigated the transcriptional changes that guide early iNKT cell development by bulk 3’-sequencing of poly(A)-RNA of induced developing iNKT cells between 12h and 5d after induction in comparison to DP thymocytes, stage 0 CD24+ iNKT cells as well as NKT1, NKT2 and NKT17 mature subsets. Briefly, CD4-CreERt2 Vα14iStopF (± Traj18KO) mice were administered with 4-OHT and sacrificed at different timepoints after administration (between 12h and 120h). 1000 cells of the following samples were FACS purified for RNA-seq analyses: 9 timepoints of induced iNKT cells (from 12h to 120h) (gated as mCD1d-PBS57- Tetramer+ CD44low NK1.1−); DP = DP thymocytes (TCRβ− mCD1d-PBS57-Tetramer− CD4+ CD8+ CD69−); CD24+ = early wildtype iNKT cells (mCD1d-PBS57-Tetramer+ CD44− CD24+); NKT1 (mCD1d-PBS57-Tetramer+ CD44+ NK1.1+ CD27+ CD138−); NKT2 (mCD1d- PBS57-Tetramer+, NK1.1− PLZFeGFP+ ICOS+ IL17RB+ CD138−); NKT17 (mCD1d- PBS57-Tetramer+ CD19− ICOS+ CD138+). For each condition, four biological replicates were sequenced (except for 96h timepoint, where two samples of the same mouse where sequenced). For the NKT17 samples and two NKT2 samples, 2 mice were pooled. For CD24+ samples, 4-6 mice were pooled. Library preparation for bulk 3’-sequencing of poly(A)-RNA was done as described previously (Parekh et al., 2016). The library was sequenced on a NextSeq 500 (Illumina) with 16 cycles for the barcodes and UMIs and 50 cycles for the cDNA.

: Project data type: Other

Scope:: Monoisolate

Klinikum rechts der Isar, II. Medizinische Klinik

Accession:: PRJEB46388
ID:: 763452

Select item 6630733.

KUGBM8 GBM primary cell lines

Oncopanel testing capture was carried out with Illumina’s TruSight Cancer Kit, which screens 94 genes and 284SNPs (Illumina, Inc). Sequencing was performed using MiSeq sequencer (Illumina) to produce 2 × 150 bp reads. Raw reads were mapped against reference genome hg19 using bwa-mem, de-duplicated using Picard tools and variant calling was performed using GATK best practices pipeline. Quality filtered variants (minimum of × 20 coverage and no more than 10% MAPQ0) were annotated using ANNOVAR with avSNP release of 142, 1000 genomes release of 2014 along with NIH-NHLBI 6500 exome database version 2. MAF filter (<0.01) was set based on ExAC, NIH 6500, gnomAD and 1000 genomes data. Pathogenicity evaluation was performed based on the inheritance mode, database entries (HGMD, ClinVar, CentoMD), in silico prediction tools (SIFT, Polyphen2, MutationTaster) and ACMG recommendations. All intronic variants located outside the boundaries of 10 bp from the exons and synonymous exonic ones were filtered out. Putative splicing variants were analyzed using Human Splicing Finder (HSF).