BioProject

Peanut allergy reaction severity correlates with increased intestinal epithelial cell (IEC) barrier permeability. More...

Peanut allergy reaction severity correlates with increased intestinal epithelial cell (IEC) barrier permeability. CC027/GeniUnc mice develop peanut allergy by intragastric administration of peanut proteins without adjuvant. We report that peanut-allergic CC027/GeniUnc mice showed increased IEC barrier permeability and systemic peanut allergen Ara h 2 after challenge. Jejunal epithelial cell transcriptomics showed effects of peanut allergy on IEC proliferation, survival, and metabolism, and revealed IEC-predominant angiopoietin like-4 (Angptl4) as a unique feature of CC027/GeniUnc peanut allergy. Peanut-allergic pediatric patients demonstrated significantly higher serum ANGPTL4 compared to non-peanut-allergic but atopic patients, highlighting its potential as a biomarker of peanut allergy. Overall design: 15 isolated jejunal epithelial cell samples from CC027/GeniUnc mice (n=15) representing 5 experimental conditions: PBS-sensitized & unchallenged (n=3); Peanut-sensitized & unchallenged (n=3); Peanut-sensitized & challenged, 30 minutes post-challenge (n=3); Peanut-sensitized & challenged, 60 minutes post-challenge (n=3); Peanut-sensitized & challenged, 120 minutes post-challenge (n=3). Less...