BioProject

Following fertilization, the genomes of the germ cells are reprogrammed to form the totipotent embryo. More...

Following fertilization, the genomes of the germ cells are reprogrammed to form the totipotent embryo. Pioneer transcription factors are essential for remodeling the chromatin and driving the initial wave of zygotic gene expression. In Drosophila melanogaster, the pioneer factor Zelda is essential for development through this dramatic period of reprogramming, known as the maternal-to-zygotic transition (MZT). However, it was unknown whether additional pioneer factors were required for this transition. We identified an additional maternally encoded factor required for development through the MZT, GAGA Factor (GAF). GAF is necessary to activate widespread zygotic transcription and to remodel the chromatin accessibility landscape. We demonstrated that Zelda preferentially controls expression of the earliest transcribed genes, while genes expressed during widespread activation are predominantly dependent on GAF. Thus, progression through the MZT requires coordination of multiple pioneer-like factors, and we propose that as development proceeds control is gradually transferred from Zelda to GAF. Overall design: GAF ChIP-seq was performed in duplicate using anti-GFP antibody (Abcam #ab290) on embryos in which endogenous GAF protein was tagged with super folder GFP (sfGFP). Sequencing was performed on immunoprecipitated samles as well as input controls. Additional immunoprecipitations were performed using the anti-GFP antibody in w1118 to control for nonspecific pulldown. Zelda ChIP was performed in duplicate with the anti-Zelda antibody (Harrison et al., PLoS Genetics 2011) with input controls. Less...