BioProject

To investigate dynamic change of the respiratory and gut microbiota of Covid-19 patients . More...

To investigate dynamic change of the respiratory and gut microbiota of Covid-19 patients . A total of 63 subjects, including 38 laboratory-confirmed COVID-19 patients, 10 SARS-CoV-2 negative (non-COVID-19) patients with various diseases and 15 healthy adults, were enrolled in this study. Specimens including throat swabs and anal swabs were collected from the patients during hospitalization (10-40) at Nantong Third Hospital Affiliated to Nantong University and from healthy adults when they visited the same hospital for physical examination. Bacterial DNA was extracted from the swabs using a QIAamp DNA Microbiome Kit (QIAGEN, Dusseldorf, Germany) according to the manufacturer instructions. The V4 hypervariable region (515-806 nt) of the 16S rRNA gene was amplified universal bacterial primers. Purified amplicons were paired-end sequenced (2x250) using Illumina P250 sequencer. To make it easier to split the data according to samples from all libraries, forward reads and reverse reads were merged using 'fastq_mergepairs' command in usearch(v11) Less...