BioProject

Background: Atopic eczema (atopic dermatitis, AD) is characterized by disrupted skin barrier associated with elevated skin pH and skin microbiome dysbiosis, due to high Staphylococcus aureus loads, especially during flares. More...

Background: Atopic eczema (atopic dermatitis, AD) is characterized by disrupted skin barrier associated with elevated skin pH and skin microbiome dysbiosis, due to high Staphylococcus aureus loads, especially during flares. Since S. aureus shows optimal growth at neutral pH, we investigated the longitudinal interplay between these factors and AD severity. Method: Emollient (with either basic pH 8.5 or pH 5.5) was applied double blinded twice daily to 6 AD patients and 6 healthy (HE) controls for 8 weeks. Weekly, skin swabs for microbiome analysis (deep sequencing) were taken, AD severity assessed and skin physiology (pH, hydration, transepidermal water loss) measured.Results: Physiological, microbiome and clinical results were not robustly related to the pH of applied emollient. In contrast to longitudinally stable microbiome in HE, S. aureus frequency significantly increased in AD over 8 weeks. High S. aureus abundance was associated with skin pH 5.7-6.2. High baseline S. aureus frequency predicted both increase in S. aureus and in AD severity (EASI and local SCORAD) after 8 weeks. Conclusion: Skin pH is tightly regulated by intrinsic factors and limits the abundance of S. aureus. High baseline S. aureus abundance in turn predicts an increase in AD severity over the study period. This underlines the importance and potential of sustained intervention regarding the skin pH and urges for larger studies linking skin pH and skin S. aureus abundance to understand driving factors of disease progression. Less...